IVbteA Modified Colorimetric MTT Assay Adapted fbr Application to Proliferation and Cytotoxicity Assays

to a culture base, their cell-to-cell interactions become very tight, so that they cannot be dispersed perfectly by the convcntional techniques (e.g,, trypsin,iEDTA treatment),i'!) To resolve this problem, we tried to apply a colorimetric MTT assay to primary cultured hepatocytes. The MTT assay is a rapid colorimetric microtiter assay which was originally developed to measure the living cell number ef lyrnphocytes.3) This methed is based on t-he selective ability of mitochondrial dehydrogenases in living cells to reduce the yellow soluble salt MTT (3-(4.5-dirnethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromidc)toapurple insoluble formuzun precipitate, The number of viable cells is, therefore,